|
KMID : 0370220190630060351
|
|
Yakhak Hoeji
2019 Volume.63 No. 6 p.351 ~ p.358
|
|
|
Analytical Method Development of Cholic Acid in Human Plasma and Gall Bladder Bile by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
|
|
Na Seong-Hoon
Yoon Hye-Ran
|
|
|
Abstract
|
|
|
Bile acids are increasingly appreciated as bioactive molecules and important end products of cholesterolmetabolism. While they have been identified as key factors in lipid emulsification and absorption due to their detergentproperties. bile acids have also been shown to act as signaling molecules and intermediates between the host and the gutmicrobiota. To investigate bile acid functions in humans, an advanced platform for high throughput analysis is essential.
Herein, we developed the analytical method of cholic acids following simple one step protein precipitation from biologicalsample by UPLC-MS/MS. MRM ions was m/z=407.2 for cholic acid. The R2 of calibration curves provided 0.9995 in thecalibration range of 0.005~5 μg/mL. The quantification system was validated with excellent sensitivity that allowsquantitative targeted analyses of bile acids. The LOD (0.001 μg/mL), LOQ (0.005 μg/mL), recoveries (96.8~101.3%±2.7~4.0%) on intra-day assay, and (98.4~111.0%±2.3~3.4%) on inter-day assay achieved resonable validated data for bileacids analyses. The developed method was applied for drawing plasma and bile acid profile in both normal and diseasestatus. Our study is characterized by rapid and simple sample preparation as well as successful application to plasma andbile. These results could be usable for routine diagnostic monitoring of bile acids on human biofluids.
|
|
|
KEYWORD
|
|
|
bile acids, cholic acid, stable isotope, deproteinization, UPLC-MS/MS, plasma, gall bladder
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|